APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 , HeLa , Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat2
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr407 of human FAK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886612
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK
Cellular Localization
Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side
Background
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX132320 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (GTX132320) diluted at 1:500.
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GTX132320 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (GTX132320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132320 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (GTX132320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132320 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr407) antibody (GTX132320) diluted at 1:1000.
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GTX132320 ICC/IF Image
FAK (phospho Tyr407) antibody detects FAK (phospho Tyr407) protein at focal adhesion and nucleus by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FAK (phospho Tyr407) stained by FAK (phospho Tyr407) antibody (GTX132320) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Blue: Hoechst 33342 staining.
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REFERENCE
There are currently no references for FAK (phospho Tyr407) antibody (GTX132320). Be the first to share your publications with this product.
REVIEW
FAK (phospho Tyr407) antibody
Cat. No. GTX132320
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 22-Feb-2017
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
ARPE-19
Blocking :
3% BSA and 0.02% Triton-X100 in PBS, 25°C, 1Hr
Primary Antibody :
1:50, 4°C, 16Hr
