FAK (phospho Tyr576) antibody [HL127]

Cat. No. GTX635714
Cat. No.  GTX635714
Recombinant antibody KO/KD Orthogonal Overexpression Carrier-Free
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
GTX635714 WB Image
KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (GTX635714) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
GTX635714 WB Image
Orthogonal

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (GTX635714) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

2 / 6
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
GTX635714 WB Image
Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (GTX635714) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

3 / 6
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
GTX635714 WB Image
Orthogonal

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (GTX635714) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

4 / 6
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
GTX635714 WB Image
Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (GTX635714) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

5 / 6
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
GTX635714 WB Image
Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (GTX635714) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

6 / 6
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
Anti-FAK (phospho Tyr576) antibody [HL127] used in Western Blot (WB). GTX635714
This FAK (phospho Tyr576) antibody [HL127] (GTX635714) is a rabbit monoclonal antibody validated for detection of FAK (phospho Tyr576) by WB. Reactivity has been shown in Human, Mouse, and Rat. Knockout/knockdown validation supports antibody specificity.
  • Host
    Rabbit
  • Clonality
    Monoclonal
  • Clone Name
    HL127
  • Isotype
    IgG
  • Applications
    WB
  • Reactivity
    Human, Mouse, Rat
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