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FAK (phospho Tyr576) antibody

Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140
Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140
Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140
Anti-FAK (phospho Tyr576) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132140
Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140
Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140

Cat No. GTX132140

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat2 , HeLa , 293T , 293T treated 50ng/ml EGF , HepG2 , HepG2 treated 200ng/ml EGF

Predict Reactivity

Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human FAK (phospho Tyr576). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK

Cellular Localization

Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140

GTX132140 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.

Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140

GTX132140 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. (CIP: Calf-intestinal alkaline phosphatase)

Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140

GTX132140 WB Image

FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein by western blot analysis. Left: Untreated (-) and EGF-treated (+, 50 ng/ml for 30 min) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE. Right: Untreated (-) and EGF-treated (+, 200 ng/ml for 30 min) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE. The membranes were blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:3000.

Anti-FAK (phospho Tyr576) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132140

GTX132140 ICC/IF Image

FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FAK (phospho Tyr576) protein stained by FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140

GTX132140 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FAK (phospho Tyr576) antibody used in Western Blot (WB). GTX132140

GTX132140 WB Image

Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW

FAK (phospho Tyr576) antibody Cat No. GTX132140

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 21-Sep-2016
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : ARPE-19 cells
Blocking : 3% BSA and 0.02% Triton-X100 in PBS, 25°C, 1Hr
Primary Antibody : 1:50, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169