APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T treated 50ng/ml EGF , HepG2 treated 200ng/ml EGF , HeLa , Neuro2A , C8D30 , NIH-3T3 , C2C12 , Rat2
Predict Reactivity
Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.89 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr576 of human FAK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886575
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK
Cellular Localization
Cell junction , focal adhesion , Cell membrane; Peripheral membrane protein; Cytoplasmic side
Background
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX132140 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.
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GTX132140 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132140 IHC-P Image
FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat lung.FAK (phospho Tyr576) stained by FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX132140 ICC/IF Image
FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein at cell membrane and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FAK (phospho Tyr576) protein stained by FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX132140 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132140 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132140 WB Image
FAK (phospho Tyr576) antibody detects FAK (phospho Tyr576) protein by western blot analysis. Left: Untreated (-) and EGF-treated (+, 50 ng/ml for 30 min) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE. Right: Untreated (-) and EGF-treated (+, 200 ng/ml for 30 min) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE. The membranes were blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:3000.
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GTX132140 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132140 WB Image
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132140 WB Image
Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody (GTX132140) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for FAK (phospho Tyr576) antibody (GTX132140). Be the first to share your publications with this product.
REVIEW
FAK (phospho Tyr576) antibody
Cat. No. GTX132140
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Rating
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( Average 4 based on 1 users reviews)
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Application
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 21-Sep-2016
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
ARPE-19 cells
Blocking :
3% BSA and 0.02% Triton-X100 in PBS, 25°C, 1Hr
Primary Antibody :
1:50, 4°C, 16Hr
