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FAK (phospho Tyr576) antibody

Cat. No. GTX24815

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse, Xenopus laevis
Package
50 μl ($399)
See all FAK products

APPLICATION

Application Note

WB: Use at a concentration of 0.1 - 0.5 μg/ml. Predicted molecular weight: 125 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

119 kDa. ( Note )

Positive Control

Chicken embryo fibroblasts expressing Focal Adhesion Kinase protein and plated on fibronectin

Product Note

Some cross-reactivity with PYK 2 may still be experienced in cases where PYK 2 is overexpressed relative to Focal Adhesion Kinase within the same cell system.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1mg/ml BSA (IgG, Protease free)

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (Human) derived from the region of human Focal Adhesion Kinase that contains tyrosine 576. The sequence is conserved in mouse, rat, chicken and frog.

Purification

Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine kinase 2 , FADK , FAK , FAK1 , FRNK , PPP1R71 , p125FAK , pp125FAK

Cellular Localization

Cytoplasm, cytoskeleton , Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399