FAK (phospho Tyr576) antibody

Cat No. GTX32250

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Rat

See all FAK products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:1000

Not tested in other applications.

Calculated MW

119 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of FAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Protein Tyrosine Kinase 2,Fadk,Fak,Fak1,Frnk,Ppp1R71,P125Fak,Pp125Fak,Ptk2

Cellular Localization

Cell cortex,Cell junction,Cell membrane,Centrosome,Cytoplasm,Cytoskeleton,Focal adhesion,Microtubule organizing center,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]

Database

Gene ID: 5747
UniProt: Q05397

Research Area

DATA IMAGES

GTX32250 WB Image

WB analysis of Forskolin-treated HEK293T (A), Forskolin-treated NIH3T3 (B), Forskolin-treated H9C2 (C) whole cell lysates using GTX32250 FAK (phospho Tyr576) antibody.