GeneTex
United States (US)

FAK (phospho Tyr925) antibody

GTX32252_20190131_WB_19013101_519.jpg
GTX32252_20190131_ICCIF_19013102_796.jpg

Cat No. GTX32252

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
Not tested in other applications.

Calculated MW

119 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of FAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Tyrosine Kinase 2,Fadk,Fak,Fak1,Frnk,Ppp1R71,P125Fak,Pp125Fak,Ptk2

Cellular Localization

Cell cortex,Cell junction,Cell membrane,Centrosome,Cytoplasm,Cytoskeleton,Focal adhesion,Microtubule organizing center,Nucleus

Background

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]

Database

Research Area

DATA IMAGES
GTX32252_20190131_WB_19013101_519.jpg

GTX32252 WB Image

WB analysis of pervanadate-treated NIH3T3 (A) whole cell lysates using GTX32252 FAK (phospho Tyr925) antibody.

GTX32252_20190131_ICCIF_19013102_796.jpg

GTX32252 ICC/IF Image

ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX32252 FAK (phospho Tyr925) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Package List Price ($)
$ 319