Immunohistochemical analysis of paraffin-embedded human breast cancer, using FAM120A(GTX120824) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

FAM120A antibody detects FAM120A protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FAM120A protein stained by FAM120A antibody (GTX120824) diluted at 1:500.
Blue: Hoechst 33343 staining.

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX120824 diluted at 1:1000