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FAM120A antibody

Anti-FAM120A antibody used in Western Blot (WB). GTX120824
Anti-FAM120A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120824
Anti-FAM120A antibody used in IHC (Paraffin sections) (IHC-P). GTX120824

Cat. No. GTX120824

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FAM120A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FAM120A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723014

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

family with sequence similarity 120A , C9orf10 , HBVPTPAP , OSSA

Cellular Localization

Cytoplasm , Cell membrane; Peripheral membrane protein; Cytoplasmic side

Background

May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.

Database

Research Area

DATA IMAGES

Anti-FAM120A antibody used in Western Blot (WB). GTX120824

GTX120824 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
7.5% SDS PAGE
GTX120824 diluted at 1:1000

Anti-FAM120A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX120824

GTX120824 ICC/IF Image

FAM120A antibody detects FAM120A protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FAM120A protein stained by FAM120A antibody (GTX120824) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-FAM120A antibody used in IHC (Paraffin sections) (IHC-P). GTX120824

GTX120824 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using FAM120A(GTX120824) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169