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FAN antibody [N2C1], Internal

Anti-FAN antibody [N2C1], Internal used in Western Blot (WB). GTX103723
Anti-FAN antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX103723

Cat No. GTX103723

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

FAN tansfected 293T cell

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FAN. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

neutral sphingomyelinase activation associated factor , FAN , GRAMD5

Background

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES
Anti-FAN antibody [N2C1], Internal used in Western Blot (WB). GTX103723

GTX103723 WB Image

Western Blot analysis of NSMAF expression in transfected 293T cell line by NSMAF polyclonal antibody.
A: Non-transfected lysate.
B: NSMAF transfected lysate.
7.5% SDS PAGE
GTX103723 diluted at 1:500

Anti-FAN antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX103723

GTX103723 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human T(gastric cancer) , using NSMAF(GTX103723) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for FAN antibody [N2C1], Internal (GTX103723). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FAN antibody [N2C1], Internal (GTX103723). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169