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FANCA antibody [N1], N-term

Anti-FANCA antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113433
Anti-FANCA antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX113433

Cat. No. GTX113433

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FANCA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

163 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FANCA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036897

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FA complementation group A , FA , FA-H , FA1 , FAA , FACA , FAH , FANCH

Cellular Localization

Nucleus , Cytoplasm

Background

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are the most common cause of Fanconi anemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FANCA antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113433

GTX113433 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FACA(GTX113433) antibody at 1:200 dilution.

Anti-FANCA antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX113433

GTX113433 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OV90 xenograft, using FACA(GTX113433) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Truong LN et al. J Biol Chem 2014; 289 (42) : 28910-23 Homologous recombination is a primary pathway to repair DNA double-strand breaks generated during DNA rereplication.
Application : WB
Reactivity : Human
Kim S et al. Int J Biochem Cell Biol 2013; 45 (9) : 1953-61 Fanconi anemia complementation group A (FANCA) localizes to centrosomes and functions in the maintenance of centrosome integrity.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169