GeneTex
United States (US)

FANCC antibody [N1C1]

GTX100400_IFA_19011506_200.jpg
GTX100400_39721_WB_19011506_540.jpg
GTX100400_WB_M_19011506_469.jpg
GTX100400_39721_IHC_19011506_147.jpg

Cat No. GTX100400

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.989mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FANCC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fa Complementation Group C,Fa3,Fac,Facc,Fancc

Cellular Localization

Nucleus , Cytoplasm

Background

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group C. [provided by RefSeq]

Database

DATA IMAGES
GTX100400_IFA_19011506_200.jpg

GTX100400 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FANCC(GTX100400) antibody at 1:200 dilution.

GTX100400_39721_WB_19011506_540.jpg

GTX100400 WB Image

FANCC antibody detects FANCC protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5 % SDS-PAGE, and blotted with FANCC antibody (GTX100400) diluted by 1:1000

GTX100400_WB_M_19011506_469.jpg

GTX100400 WB Image

Sample (30 ug of whole cell lysate)

A: NIH-3T3

7.5% SDS PAGE

FANCC antibody

GTX100400 diluted at 1:1000

GTX100400_39721_IHC_19011506_147.jpg

GTX100400 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using FANCC(GTX100400) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169