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FANCD2 antibody [103]

KO/KD
Anti-FANCD2 antibody [103] used in Western Blot (WB). GTX70299
Anti-FANCD2 antibody [103] used in Western Blot (WB). GTX70299
Anti-FANCD2 antibody [103] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70299
Anti-FANCD2 antibody [103] used in Western Blot (WB). GTX70299

Cat. No. GTX70299

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 103

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all FANCD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

164 kDa. ( Note )

Positive Control

293T , HeLa

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human FANCD2 fusion protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_370447

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FA complementation group D2 , FA-D2 , FA4 , FACD , FAD , FAD2 , FANCD

Cellular Localization

Nucleus

Background

The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-FANCD2 antibody [103] used in Western Blot (WB). GTX70299

GTX70299 WB Image

KO/KD

Wild-type (WT) and FANCD2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCD2 antibody [103] (GTX70299) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-FANCD2 antibody [103] used in Western Blot (WB). GTX70299

GTX70299 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCD2 antibody [103] (GTX70299) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-FANCD2 antibody [103] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70299

GTX70299 ICC/IF Image

FANCD2 antibody [103] detects FANCD2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FANCD2 stained by FANCD2 antibody [103] (GTX70299) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000.

Anti-FANCD2 antibody [103] used in Western Blot (WB). GTX70299

GTX70299 WB Image

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations