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FANCJ antibody [N1N2], N-term

Anti-FANCJ antibody [N1N2], N-term used in Western Blot (WB). GTX100072
Anti-FANCJ antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100072
Anti-FANCJ antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100072
Anti-FANCJ antibody [N1N2], N-term used in Western Blot (WB). GTX100072

Cat. No. GTX100072

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FANCJ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human FANCJ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036368

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA1 interacting protein C-terminal helicase 1 , BACH1 , FANCJ , OF

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FANCJ antibody [N1N2], N-term used in Western Blot (WB). GTX100072

GTX100072 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-FANCJ antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100072

GTX100072 IHC-P Image

FANCJ antibody [N1N2], N-term detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
FANCJ antibody [N1N2], N-term (GTX100072) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FANCJ antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100072

GTX100072 ICC/IF Image

FANCJ antibody [N1N2], N-term detects BRIP1 protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:500.
Blue: Hoechst 33343 staining.
Anti-FANCJ antibody [N1N2], N-term used in Western Blot (WB). GTX100072

GTX100072 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169