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FANCJ antibody [N1N2], N-term

Anti-FANCJ antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100072
Anti-FANCJ antibody [N1N2], N-term used in Western Blot (WB). GTX100072
Anti-FANCJ antibody [N1N2], N-term used in Western Blot (WB). GTX100072
Anti-FANCJ antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100072

Cat No. GTX100072

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human FANCJ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036368

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BRCA1 interacting protein C-terminal helicase 1 , BACH1 , FANCJ , OF

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FANCJ antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100072

GTX100072 IHC-P Image

FANCJ antibody [N1N2], N-term detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
FANCJ antibody [N1N2], N-term (GTX100072) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FANCJ antibody [N1N2], N-term used in Western Blot (WB). GTX100072

GTX100072 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-FANCJ antibody [N1N2], N-term used in Western Blot (WB). GTX100072

GTX100072 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-FANCJ antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100072

GTX100072 ICC/IF Image

FANCJ antibody [N1N2], N-term detects BRIP1 protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:500.
Blue: Hoechst 33343 staining.

REFERENCE

There are currently no references for FANCJ antibody [N1N2], N-term (GTX100072). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FANCJ antibody [N1N2], N-term (GTX100072). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 329
$ 169