GeneTex
United States (US)

FANCJ antibody [N1N2], N-term

GTX100072_39384_IHC_19011506_749.jpg
GTX100072_39384_IFA_19011506_394.jpg
GTX100072_WB_19011506_640.jpg

Cat No. GTX100072

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

HeLa
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human FANCJ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BACH1 antibody, FANCJ antibody, FLJ90232 antibody, MGC126521 antibody, MGC126523 antibody, OF antibody, BRIP1 antibody, Fanconi anemia group J protein antibody, BRCA1/BRCA2-associated helicase 1 antibody, BRCA1-binding helicase-like protein BACH1 antibody, BRCA1-associated C-terminal helicase 1 antibody, ATP-dependent RNA helicase BRIP1 antibody, BRCA1 interacting protein C-terminal helicase 1 antibody

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq]

Database

DATA IMAGES
GTX100072_39384_IHC_19011506_749.jpg

GTX100072 IHC-P Image

FANCJ antibody [N1N2], N-term detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SAS xenograft.
FANCJ antibody [N1N2], N-term (GTX100072) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100072_39384_IFA_19011506_394.jpg

GTX100072 ICC/IF Image

FANCJ antibody [N1N2], N-term detects BRIP1 protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term (GTX100072) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX100072_WB_19011506_640.jpg

GTX100072 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
5% SDS PAGE
FANCJ antibody
GTX100072 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169