GeneTex
  • Country / Location Selection

United States (US)

FAP antibody [N1N3]

Anti-FAP antibody [N1N3] used in Western Blot (WB). GTX102732

Cat No. GTX102732

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FAP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

fibroblast activation protein alpha , DPPIV , FAPA , FAPalpha , SIMP

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Cell projection , lamellipodium membrane; Single-pass type II membrane protein , invadopodium membrane; Single-pass type II membrane protein

Background

The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-FAP antibody [N1N3] used in Western Blot (WB). GTX102732

GTX102732 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
B: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX102732 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
There are currently no reviews for FAP antibody [N1N3] (GTX102732). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169