APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Chicken, Pig, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human FAP(Accession Q12884), corresponding to amino acid residues W346-E396.
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
fibroblast activation protein alpha , DPPIV , FAPA , FAPalpha , SIMP
Cellular Localization
Cell membrane , Membrane , Cytoplasm
Background
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
Database
Research Area
DATA IMAGES
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GTX04673 IHC-P Image
IHC-P analysis of mouse colon tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of human mammary cancer tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of human normal tissues adjacent to colorectal cancer tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of human pancreatic cancer tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 WB Image
WB analysis of rat kidney tissue lysate using GTX04673 FAP antibody. The lane on the left was treated with blocking peptide.
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GTX04673 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of mouse testis tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of rat testis tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX04673 FAP antibody.
Red : Primary antibody
Green : beta actin
Blue : DAPI
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of human colorectal cancer tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of human normal tissues adjacent to esophageal cancer tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of mouse stomach tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of human normal tissues adjacent to pancreatic cancer using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of mouse ovary tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of human mammary cancer tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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GTX04673 IHC-P Image
IHC-P analysis of rat ovary tissue using GTX04673 FAP antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100
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REFERENCE
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REVIEW
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