GeneTex
United States (US)

FARSB antibody

GTX115336_40695_IHC_R_18121410_576.jpg
GTX115336_40695_IHC_18121410_471.jpg
GTX115336_40695_WB_18121410_623.jpg
GTX115336_40695_20150821_IHC-P_M_2_18121410_742.jpg
GTX115336_40695_20150821_IHC-P_M_18121410_340.jpg

Cat No. GTX115336

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

PC-3 , U87-MG , SK-N-SH

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human FARSB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phenylalanyl-Trna Synthetase Subunit Beta,Farslb,Frsb,Hspc173,Nedblla,Phehb,Phers,Farsb

Cellular Localization

Cytoplasm

Background

This gene encodes a highly conserved enzyme that belongs to the aminoacyl-tRNA synthetase class IIc subfamily. This enzyme comprises the regulatory beta subunits that form a tetramer with two catalytic alpha subunits. In the presence of ATP, this tetramer is responsible for attaching L-phenylalanine to the terminal adenosine of the appropriate tRNA. A pseudogene located on chromosome 10 has been identified. [provided by RefSeq]

Database

DATA IMAGES
GTX115336_40695_IHC_R_18121410_576.jpg

GTX115336 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using FARSB(GTX115336) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115336_40695_IHC_18121410_471.jpg

GTX115336 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using FARSB(GTX115336) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115336_40695_WB_18121410_623.jpg

GTX115336 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
B: U87-MG
C: SK-N-SH
7.5% SDS PAGE
GTX115336 diluted at 1:1000

GTX115336_40695_20150821_IHC-P_M_2_18121410_742.jpg

GTX115336 IHC-P Image

FARSB antibody detects FARSB protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
FARSB antibody (GTX115336) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX115336_40695_20150821_IHC-P_M_18121410_340.jpg

GTX115336 IHC-P Image

FARSB antibody detects FARSB protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FARSB antibody (GTX115336) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169