APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , H1299 , HeLa , HepG2 , Molt-4 , Raji
Predict Reactivity
Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1%BSA, 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.39mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human FARSLA. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CML33 antibody, FARSL antibody, FARSLA antibody, FRSA antibody, PheHA antibody, FARSA antibody, "phenylalanine tRNA ligase 1, alpha, cytoplasmic antibody", phenylalanine-tRNA synthetase alpha-subunit antibody, pheRS antibody, "phenylalanine-tRNA synthetase-like, alpha subunit antibody", phenylalanine--tRNA ligase alpha chain antibody, phenylalanyl-tRNA synthetase alpha chain antibody, "phenylalanyl-tRNA synthetase-like, alpha subunit antibody", "phenylalanyl-tRNA synthetase, alpha subunit antibody"
Cellular Localization
Cytoplasm
Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq]
Database
DATA IMAGES
|
GTX104924 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX104924 diluted at 1:1000
|
|
GTX104924 IHC-P Image
FARSLA antibody [N2C2], Internal detects FARSLA protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
FARSLA antibody [N2C2], Internal (GTX104924) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX104924 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using FARSLA (GTX104924) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX104924 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using FARSLA(GTX104924) antibody at 1:200 dilution.
|
