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FARSLA antibody [N2C2], Internal

Anti-FARSLA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104924
Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924
Anti-FARSLA antibody [N2C2], Internal used in Western Blot (WB). GTX104924
Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924

Cat. No. GTX104924

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all FARSLA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FARSLA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950255

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phenylalanyl-tRNA synthetase subunit alpha , CML33 , FARSL , FARSLA , FRSA , PheHA

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FARSLA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104924

GTX104924 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using FARSLA(GTX104924) antibody at 1:200 dilution.

Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924

GTX104924 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using FARSLA (GTX104924) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FARSLA antibody [N2C2], Internal used in Western Blot (WB). GTX104924

GTX104924 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX104924 diluted at 1:1000

Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924

GTX104924 IHC-P Image

FARSLA antibody [N2C2], Internal detects FARSLA protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
FARSLA antibody [N2C2], Internal (GTX104924) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169