GeneTex
United States (US)

FARSLA antibody [N2C2], Internal

GTX104924_WB_19011506_422.jpg
GTX104924_40093_20150506_IHC_M_19011506_353.jpg
GTX104924_IHC_19011506_554.jpg
GTX104924_40093_IFA_19011506_217.jpg

Cat No. GTX104924

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1%BSA, 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FARSLA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CML33 antibody, FARSL antibody, FARSLA antibody, FRSA antibody, PheHA antibody, FARSA antibody, "phenylalanine tRNA ligase 1, alpha, cytoplasmic antibody", phenylalanine-tRNA synthetase alpha-subunit antibody, pheRS antibody, "phenylalanine-tRNA synthetase-like, alpha subunit antibody", phenylalanine--tRNA ligase alpha chain antibody, phenylalanyl-tRNA synthetase alpha chain antibody, "phenylalanyl-tRNA synthetase-like, alpha subunit antibody", "phenylalanyl-tRNA synthetase, alpha subunit antibody"

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq]

Database

DATA IMAGES
GTX104924_WB_19011506_422.jpg

GTX104924 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX104924 diluted at 1:1000

GTX104924_40093_20150506_IHC_M_19011506_353.jpg

GTX104924 IHC-P Image

FARSLA antibody [N2C2], Internal detects FARSLA protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
FARSLA antibody [N2C2], Internal (GTX104924) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104924_IHC_19011506_554.jpg

GTX104924 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using FARSLA (GTX104924) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104924_40093_IFA_19011506_217.jpg

GTX104924 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using FARSLA(GTX104924) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169