APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , H1299 , HeLa , HepG2 , Molt-4 , Raji
Predict Reactivity
Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7), 1% BSA. 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.39 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human FARSLA. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
phenylalanyl-tRNA synthetase subunit alpha , CML33 , FARSL , FARSLA , FRSA , PheHA
Cellular Localization
Cytoplasm
Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. This gene encodes a product which is similar to the catalytic subunit of prokaryotic and Saccharomyces cerevisiae phenylalanyl-tRNA synthetases (PheRS). This gene product has been shown to be expressed in a tumor-selective and cell cycle stage- and differentiation-dependent manner, the first member of the tRNA synthetase gene family shown to exhibit this type of regulated expression [provided by RefSeq]
Database
DATA IMAGES
![Anti-FARSLA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104924](/upload/website/prouct_img/middle/GTX104924/GTX104924_40093_IFA_19011506_217.jpg "Anti-FARSLA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104924")
|
GTX104924 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using FARSLA(GTX104924) antibody at 1:200 dilution.
|
![Anti-FARSLA antibody [N2C2], Internal used in Western Blot (WB). GTX104924](/upload/website/prouct_img/middle/GTX104924/GTX104924_WB_19011506_422.jpg "Anti-FARSLA antibody [N2C2], Internal used in Western Blot (WB). GTX104924")
|
GTX104924 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX104924 diluted at 1:1000
|
![Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924](/upload/website/prouct_img/middle/GTX104924/GTX104924_40093_20150506_IHC_M_19011506_353.jpg "Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924")
|
GTX104924 IHC-P Image
FARSLA antibody [N2C2], Internal detects FARSLA protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
FARSLA antibody [N2C2], Internal (GTX104924) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
![Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924](/upload/website/prouct_img/middle/GTX104924/GTX104924_IHC_19011506_554.jpg "Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924")
|
GTX104924 IHC-P Image
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using FARSLA (GTX104924) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
REFERENCE
There are currently no references for FARSLA antibody [N2C2], Internal (GTX104924). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FARSLA antibody [N2C2], Internal (GTX104924). Be the first to share your experience with this product.
![Anti-FARSLA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104924](/upload/website/prouct_img/small/GTX104924/GTX104924_40093_IFA_19011506_217.jpg "Anti-FARSLA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104924")
![Anti-FARSLA antibody [N2C2], Internal used in Western Blot (WB). GTX104924](/upload/website/prouct_img/small/GTX104924/GTX104924_WB_19011506_422.jpg "Anti-FARSLA antibody [N2C2], Internal used in Western Blot (WB). GTX104924")
![Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924](/upload/website/prouct_img/small/GTX104924/GTX104924_40093_20150506_IHC_M_19011506_353.jpg "Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924")
![Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924](/upload/website/prouct_img/small/GTX104924/GTX104924_IHC_19011506_554.jpg "Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924")
![Anti-FARSLA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104924](/upload/website/prouct_img/normal/GTX104924/GTX104924_40093_IFA_19011506_217.jpg "Anti-FARSLA antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104924")
![Anti-FARSLA antibody [N2C2], Internal used in Western Blot (WB). GTX104924](/upload/website/prouct_img/normal/GTX104924/GTX104924_WB_19011506_422.jpg "Anti-FARSLA antibody [N2C2], Internal used in Western Blot (WB). GTX104924")
![Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924](/upload/website/prouct_img/normal/GTX104924/GTX104924_40093_20150506_IHC_M_19011506_353.jpg "Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924")
![Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924](/upload/website/prouct_img/normal/GTX104924/GTX104924_IHC_19011506_554.jpg "Anti-FARSLA antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104924")
