GeneTex

  • Country / Location Selection

United States (US)

FAST antibody

Anti-FAST antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102571
Anti-FAST antibody used in IHC (Paraffin sections) (IHC-P). GTX102571
Anti-FAST antibody used in Western Blot (WB). GTX102571
Anti-FAST antibody used in Western Blot (WB). GTX102571
Anti-FAST antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102571
Anti-FAST antibody used in Western Blot (WB). GTX102571

Cat. No. GTX102571

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FAST products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , NIH-3T3 , PC-12

Predict Reactivity

Bovine, Dog, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human FAST. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1950259

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fas activated serine/threonine kinase , FAST

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FAST antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102571

GTX102571 ICC/IF Image

FAST antibody detects FAST protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FAST protein stained by FAST antibody (GTX102571) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-FAST antibody used in IHC (Paraffin sections) (IHC-P). GTX102571

GTX102571 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using FAST(GTX102571) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FAST antibody used in Western Blot (WB). GTX102571

GTX102571 WB Image

FAST antibody detects FASTK protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
FAST antibody (GTX102571) dilution: 1:1000

Anti-FAST antibody used in Western Blot (WB). GTX102571

GTX102571 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX102571 diluted at 1:1000

Anti-FAST antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102571

GTX102571 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using FAST (GTX102571) antibody at 1:200 dilution.

Anti-FAST antibody used in Western Blot (WB). GTX102571

GTX102571 WB Image

FAST antibody detects FASTK protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
E. 30 μg A375 whole cell lysate/extract
7.5 % SDS-PAGE
FAST antibody (GTX102571) dilution: 1:1000

REFERENCE

There are currently no references for FAST antibody (GTX102571). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for FAST antibody (GTX102571). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169