GeneTex
United States (US)

FAST antibody

GTX102571_39778_WB_19011506_532.jpg
GTX102571_39778_IFA_19011506_432.jpg
GTX102571_IFA_19011506_130.jpg
GTX102571_39778_IHC_19011506_496.jpg
GTX102571_39778_WB_R_19011506_605.jpg
GTX102571_WB_M_19011506_853.jpg

Cat No. GTX102571

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , NIH-3T3 , PC-12

Predict Reactivity

Bovine, Dog, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

6.56mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human FAST. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FAST antibody, FASTK antibody, FAST kinase antibody, fas-activated serine/threonine kinase antibody

Background

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq]
Show More +

Database

Research Area

DATA IMAGES
GTX102571_39778_WB_19011506_532.jpg

GTX102571 WB Image

FAST antibody detects FASTK protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
7.5 % SDS-PAGE
FAST antibody (GTX102571) dilution: 1:1000

GTX102571_39778_IFA_19011506_432.jpg

GTX102571 ICC/IF Image

FAST antibody detects FAST protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: FAST protein stained by FAST antibody (GTX102571) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX102571_IFA_19011506_130.jpg

GTX102571 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using FAST (GTX102571) antibody at 1:200 dilution.

GTX102571_39778_IHC_19011506_496.jpg

GTX102571 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using FAST(GTX102571) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102571_39778_WB_R_19011506_605.jpg

GTX102571 WB Image

FAST antibody detects FASTK protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
7.5 % SDS-PAGE
FAST antibody (GTX102571) dilution: 1:1000

GTX102571_WB_M_19011506_853.jpg

GTX102571 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
7.5% SDS PAGE

GTX102571 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf