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FBL4 antibody [N1C1]

Anti-FBL4 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115953
Anti-FBL4 antibody [N1C1] used in Western Blot (WB). GTX115953
Anti-FBL4 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115953

Cat No. GTX115953

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , human FBXL4-transfected 293T cells

Predict Reactivity

Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FBL4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

F-box and leucine rich repeat protein 4 , FBL4 , FBL5 , MTDPS13

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 9 tandem leucine-rich repeats. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FBL4 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX115953

GTX115953 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using FBL4(GTX115953) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FBL4 antibody [N1C1] used in Western Blot (WB). GTX115953

GTX115953 WB Image

FBL4 antibody [N1C1] detects FBL4 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human FBXL4-transfected 293T cells
7.5 % SDS-PAGE
FBL4 antibody [N1C1] (GTX115953) dilution: 1:1000

Anti-FBL4 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115953

GTX115953 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FBL4(GTX115953) antibody at 1:200 dilution.

REFERENCE

There are currently no references for FBL4 antibody [N1C1] (GTX115953). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FBL4 antibody [N1C1] (GTX115953). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf