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FBXL10 antibody [6F6G11]

Overexpression
Anti-FBXL10 antibody [6F6G11] used in Western Blot (WB). GTX04628
Anti-FBXL10 antibody [6F6G11] used in Western Blot (WB). GTX04628
Anti-FBXL10 antibody [6F6G11] used in Flow cytometry (FACS). GTX04628
Anti-FBXL10 antibody [6F6G11] used in ELISA (ELISA). GTX04628
Anti-FBXL10 antibody [6F6G11] used in Western Blot (WB). GTX04628

Cat. No. GTX04628

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6F6G11

Isotype

 IgG2b

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μl ($429)
See all FBXL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
FACS 1:200-1:400
ELISA 1:10000
Not tested in other applications.

Calculated MW

153 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human FBXL10 (AA: 457-555) expressed in E. Coli.

Purification

Purified IgG2b

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysine demethylase 2B , CXXC2 , FBXL10 , Fbl10 , JHDM1B , PCCX2

Cellular Localization

Nucleolus

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FBXL10 antibody [6F6G11] used in Western Blot (WB). GTX04628

GTX04628 WB Image

WB analysis of human FBXL10 (AA: 457-555) recombinant protein using GTX04628 FBXL10 [6F6G11].

Anti-FBXL10 antibody [6F6G11] used in Western Blot (WB). GTX04628

GTX04628 WB Image

Overexpression

WB analysis of HEK293 cells overexpressed with/without FBXL10 (AA: 457-555)-hIgGFc using GTX04628 FBXL10 antibody [6F6G11].

Anti-FBXL10 antibody [6F6G11] used in Flow cytometry (FACS). GTX04628

GTX04628 FACS Image

FACS analysis of HeLa cells using GTX04628 FBXL10 antibody [6F6G11].
Green : Primary antibody
Red : Negative control

Anti-FBXL10 antibody [6F6G11] used in ELISA (ELISA). GTX04628

GTX04628 ELISA Image

ELISA analysis of antigen using serially diluted GTX04628 FBXL10 antibody [6F6G11].
Black : Control antigen 100 ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-FBXL10 antibody [6F6G11] used in Western Blot (WB). GTX04628

GTX04628 WB Image

WB analysis of A549 whole cell lysate using GTX04628 FBXL10 antibody [6F6G11].

REFERENCE

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REVIEW

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Package List Price ($)
$ 429