APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
4-6μg/ml |
Note :
IHC-P
Human Lung shows staining of ciliated epithelial cells in the bronchioles.
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Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX25305-PEP).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence RACPKESMDWWM, from the C Terminus of the protein sequence according to NP_060173.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
F-box and leucine rich repeat protein 12 , Fbl12
Background
Members of the F-box protein family, such as FBXL12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
Database
Research Area
DATA IMAGES
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GTX25305 IHC-P Image
IHC-P analysis of human lung tissue using GTX25305 FBXL12 antibody, C-term. Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6 Dilution : 4μg/ml
Antigen retrieval : Heat induced antigen retrieval with citrate buffer pH 6 Dilution : 4μg/ml
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REFERENCE
There are currently no references for FBXL12 antibody, C-term (GTX25305). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FBXL12 antibody, C-term (GTX25305). Be the first to share your experience with this product.