GeneTex
United States (US)

FBXL12 antibody [C2C3], C-term

GTX104870_WB_M_19011506_818.jpg
GTX104870_39708_IHC_19011506_246.jpg
GTX104870_WB_19011506_190.jpg

Cat No. GTX104870

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FBXL12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ20188 antibody, Fbl12 antibody, FBXL12 antibody, F-box/LRR-repeat protein 12 antibody, F-box protein FBL12 antibody, F-box and leucine-rich repeat protein 12 antibody

Background

Members of the F-box protein family, such as FBXL12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

Database

DATA IMAGES
GTX104870_WB_M_19011506_818.jpg

GTX104870 WB Image

Sample (30 ug of whole cell lysate)

A: NIH-3T3

12% SDS PAGE

FBXL12 antibody

GTX104870 diluted at 1:1000

GTX104870_39708_IHC_19011506_246.jpg

GTX104870 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using FBXL12(GTX104870) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104870_WB_19011506_190.jpg

GTX104870 WB Image

Sample (30 ug of whole cell lysate)

A: Hep G2 (GTX27900)

10% SDS PAGE

GTX104870 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169