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FBXL12 antibody [C2C3], C-term

Anti-FBXL12 antibody [C2C3], C-term used in Western Blot (WB). GTX104870
Anti-FBXL12 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104870
Anti-FBXL12 antibody [C2C3], C-term used in Western Blot (WB). GTX104870

Cat. No. GTX104870

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all FBXL12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

NIH-3T3 , HepG2

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FBXL12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240801

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F-box and leucine rich repeat protein 12 , Fbl12

Background

Members of the F-box protein family, such as FBXL12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FBXL12 antibody [C2C3], C-term used in Western Blot (WB). GTX104870

GTX104870 WB Image

Sample (30 ug of whole cell lysate)

A: Hep G2 (GTX27900)

10% SDS PAGE

GTX104870 diluted at 1:1000

Anti-FBXL12 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104870

GTX104870 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using FBXL12(GTX104870) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FBXL12 antibody [C2C3], C-term used in Western Blot (WB). GTX104870

GTX104870 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FBXL12 antibody [C2C3], C-term (GTX104870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

There are currently no reviews for FBXL12 antibody [C2C3], C-term (GTX104870). Be the first to share your experience with this product.
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169