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FBXL2 antibody, N-term

Anti-FBXL2 antibody, N-term used in Western Blot (WB). GTX17018
Anti-FBXL2 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX17018

Cat. No. GTX17018

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μg ($399)
See all FBXL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 5-10μg/ml

Note :

IHC-P
Human Cerebral Cortex shows cytoplasm staining in neuronal bodies.
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX17018-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence VFSNNDEGLINKK-C, from the N Terminus of the protein sequence according to NP_036289.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F-box and leucine rich repeat protein 2 , FBL2 , FBL3

Cellular Localization

Membrane

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-FBXL2 antibody, N-term used in Western Blot (WB). GTX17018

GTX17018 WB Image

WB analysis of human frontal cortex (A) and rat (B) brain lysate using GTX17018 FBXL2 antibody, N-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-FBXL2 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX17018

GTX17018 IHC-P Image

IHC-P analysis of human cerebral cortex using GTX17018 FBXL2 antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 10μg/ml

REFERENCE

Application Reference

O'Brien ME et al. Mol Cell Biol 2020; 40 (12) Tumor Necrosis Factor Alpha Regulates Skeletal Myogenesis by Inhibiting SP1 Interaction with cis-Acting Regulatory Elements within the Fbxl2 Gene Promoter.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399