GeneTex
United States (US)

FBXL2 antibody [N1], N-term

GTX104864_IFA_19011506_761.jpg
GTX104864_39708_IHC_19011506_676.jpg
GTX104864_WB_19011506_266.jpg

Cat No. GTX104864

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2 , Raji

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.819 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human FBXL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

F-Box And Leucine Rich Repeat Protein 2,Fbl2,Fbl3,Fbxl2

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. [provided by RefSeq]

Database

DATA IMAGES
GTX104864_IFA_19011506_761.jpg

GTX104864 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using FBXL2(GTX104864) antibody at 1:200 dilution.

GTX104864_39708_IHC_19011506_676.jpg

GTX104864 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using FBXL2(GTX104864) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104864_WB_19011506_266.jpg

GTX104864 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Raji
10% SDS PAGE
GTX104864 diluted at 1:2000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169