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FBXO11 antibody, Internal

Anti-FBXO11 antibody, Internal used in ChIP assay (ChIP assay). GTX46904
Anti-FBXO11 antibody, Internal used in Western Blot (WB). GTX46904
Anti-FBXO11 antibody, Internal used in Western Blot (WB). GTX46904

Cat No. GTX46904

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ChIP assay

Reactivity

Human
Package
50 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-2.5 ug/ml
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

293T , human ovary tumor

PROPERTIES

Form

Liquid

Buffer

1x PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to an Internal region of Human FBXO11

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

F-box protein 11 , FBX11 , IDDFBA , PRMT9 , UBR6 , UG063H01 , VIT1

Cellular Localization

Nucleus

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. It can function as an arginine methyltransferase that symmetrically dimethylates arginine residues, and it acts as an adaptor protein to mediate the neddylation of p53, which leads to the suppression of p53 function. This gene is known to be down-regulated in melanocytes from patients with vitiligo, a skin disorder that results in depigmentation. Polymorphisms in this gene are associated with chronic otitis media with effusion and recurrent otitis media (COME/ROM), a hearing loss disorder, and the knockout of the homologous mouse gene results in the deaf mouse mutant Jeff (Jf), a single gene model of otitis media. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-FBXO11 antibody, Internal used in ChIP assay (ChIP assay). GTX46904

GTX46904 ChIP assay Image

ChIP analysis of HCT116 cells using GTX46904 FBXO11 antibody. Use 0.5μg antibody GTX46904 FBXO11 antibody for ChIP assay. Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
(+) : Add blocking peptide
(-) : no blocking peptide

Anti-FBXO11 antibody, Internal used in Western Blot (WB). GTX46904

GTX46904 WB Image

WB analysis of human ovary tumor tissue using GTX46904 FBXO11 antibody at 1μg/ml.

Anti-FBXO11 antibody, Internal used in Western Blot (WB). GTX46904

GTX46904 WB Image

WB analysis of 293T cells using GTX46904 FBXO11 antibody at 0.2-1μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369