APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , H1299 , HepG2
Predict Reactivity
Mouse, Rat, Bovine, Guinea pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FBXO2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
FBG1 antibody, FBX2 antibody, Fbs1 antibody, NFB42 antibody, OCP1 antibody, FBXO2 antibody, organ of Corti protein 1 antibody, F-box gene 1 antibody, F-box only protein 2 antibody, F-box protein 2 antibody
Background
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is highly similar to the rat NFB42 (neural F Box 42 kDa) protein which is enriched in the nervous system and may play a role in maintaining neurons in a postmitotic state. [provided by RefSeq]
Database
DATA IMAGES
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GTX104858 WB Image
Sample (30 ug of whole cell lysate)
A: H1299
12% SDS PAGE
GTX104858 diluted at 1:1000
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GTX104858 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human adrenal gland tumor, using FBXO2(GTX104858) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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