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FBXO21 antibody

Anti-FBXO21 antibody used in Western Blot (WB). GTX121415
Anti-FBXO21 antibody used in Western Blot (WB). GTX121415

Cat No. GTX121415

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

U87-MG , Rat2 , *mouse FBXO21 transfected HEK293T cells

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FBXO21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731589

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

F-box protein 21 , FBX21

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FBXO21 antibody used in Western Blot (WB). GTX121415

GTX121415 WB Image

FBXO21 antibody detects FBXO21 protein by Western blot analysis.
A. 30 μg Rat-2 whole cell lysate/extract
7.5 % SDS-PAGE
FBXO21 antibody (GTX121415) dilution: 1:1000

Anti-FBXO21 antibody used in Western Blot (WB). GTX121415

GTX121415 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX121415 diluted at 1:2000

REFERENCE

REVIEW

FBXO21 antibody Cat No. GTX121415

Rating ( Average 4 based on 1 users reviews)
Application
Immunoprecipitation(IP) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 01-Jan-2000
Score :
Application Tested : IP
Sample Species : Ms
Sample : mouse FBXO21 transfected HEK293T cells
Amount used : 20μg
Blocking : 2% skim milk/ 0.5% BSA in PBS/0.01% Tween 20, 4°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : A:Mock transfection - IP with flag, B:Flag-tagged FBXO21 -IP flag, C:Flag tagged FBXO21- IP with GTX121415, D:Fermentag pageruler plus prestained marker, E:Input control IP A, F:Input control IP B, G:Input control IP C, H:Total lysate mock transfected HEK 293T
Figure Note : 2.375 ug for IP
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169