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FBXO22 antibody [N3C3]

Anti-FBXO22 antibody [N3C3] used in Western Blot (WB). GTX117774

Cat. No. GTX117774

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FBXO22 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FBXO22. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619633

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F-box protein 22 , FBX22 , FISTC1

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Two transcript variants encoding different isoforms exist for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FBXO22 antibody [N3C3] used in Western Blot (WB). GTX117774

GTX117774 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX117774 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Nakagawa S et al. Breast Cancer Res Treat 2024; 204 (3) : 453-463 Potential role of Fbxo22 in resistance to endocrine therapy in breast cancer with invasive lobular carcinoma.
S Nakagawa et al. Research Square 2023; Potential role of Fbxo22 in resistance to endocrine therapy in breast cancer with invasive lobular carcinoma
Application : IHC-P
Daniele Simoneschi et al. Nature 2021; 592 (7856) : 789-793 CRL4AMBRA1 is a master regulator of D-type cyclins.
Application : WB
Reactivity : Human
Lignitto L et al. Cell 2019; 178 (2) : 316-329.e18 Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1.
Application : WB
Reactivity : Human
Johmura Y et al. J Clin Invest 2018; 128 (12) : 5603-5619 Fbxo22-mediated KDM4B degradation determines selective estrogen receptor modulator activity in breast cancer.
Application : IHC-P
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169