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FBXO28 antibody

Anti-FBXO28 antibody used in Western Blot (WB). GTX118254
Anti-FBXO28 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118254

Cat. No. GTX118254

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FBXO28 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

A549 , H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FBXO28. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173731

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

F-box protein 28 , CENP-30 , Fbx28

Background

Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-FBXO28 antibody used in Western Blot (WB). GTX118254

GTX118254 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
10% SDS PAGE
GTX118254 diluted at 1:1000

Anti-FBXO28 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118254

GTX118254 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FBXO28(GTX118254) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169