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FBXO32 antibody

Anti-FBXO32 antibody used in Western Blot (WB). GTX47819
Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819
Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819
Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

Cat No. GTX47819

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP, ELISA, IHC

Reactivity

Mouse, Rat
Package
100 μg ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
IHC-P Assay dependent
IP 1:200
ELISA 1:10000
IHC 1:50-1:150
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine pH 7.4-7.9, 30% glycerol, BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64-0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide taken within amino acid region 240-280 on Atr1 protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_11173218

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

F-box protein 32 , Fbx32 , MAFbx

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

GTX47819 WB Image

WB analysis of Fbx32 recombinant protein using GTX47819 FBXO32 antibody.
Dilution : 1:500

Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819

GTX47819 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX47819 FBXO32 antibody.
Dilution : 1:50

Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819

GTX47819 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX47819 FBXO32 antibody.
Dilution : 1:50

Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

GTX47819 WB Image

WB analysis of mouse heart tissue lysate using GTX47819 FBXO32 antibody.
Dilution : 1:500

REFERENCE

REVIEW

FBXO32 antibody Cat No. GTX47819

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomed Res Int in 2018.PMID: 29992158
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biomed Res Int in 2018.PMID: 29992158
Package List Price ($)
$ 409