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FBXO32 antibody

Anti-FBXO32 antibody used in Western Blot (WB). GTX47819
Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819
Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819
Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

Cat No. GTX47819

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP, ELISA

Reactivity

Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
IHC-P Assay dependent
IP 1:200
ELISA 1:10000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine pH 7.4-7.9, 30% glycerol, BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64-0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide taken within amino acid region 240-280 on Atr1 protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

F-box protein 32 , Fbx32 , MAFbx

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Database

Research Area

DATA IMAGES

Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

GTX47819 WB Image

WB analysis of Fbx32 recombinant protein using GTX47819 FBXO32 antibody.
Dilution : 1:500

Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819

GTX47819 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX47819 FBXO32 antibody.
Dilution : 1:50

Anti-FBXO32 antibody used in IHC (Paraffin sections) (IHC-P). GTX47819

GTX47819 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX47819 FBXO32 antibody.
Dilution : 1:50

Anti-FBXO32 antibody used in Western Blot (WB). GTX47819

GTX47819 WB Image

WB analysis of mouse heart tissue lysate using GTX47819 FBXO32 antibody.
Dilution : 1:500

REFERENCE

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