Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using FBXO4(GTX115815) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Sample (30 ug of whole cell lysate)
A: A431
B: HeLa
10% SDS PAGE
GTX115815 diluted at 1:2000