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FBXO43 antibody [C3], C-term

Anti-FBXO43 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104871
Anti-FBXO43 antibody [C3], C-term used in Western Blot (WB). GTX104871
Anti-FBXO43 antibody [C3], C-term used in Western Blot (WB). GTX104871
Anti-FBXO43 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104871
Anti-FBXO43 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104871

Cat No. GTX104871

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

Positive Control

293T , NIH-3T3 , JC

Predict Reactivity

Rat, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FBXO43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

F-box protein 43 , EMI2 , ERP1 , FBX43

Background

Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-FBXO43 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104871

GTX104871 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using FBXO43(GTX104871) antibody at 1:200 dilution.

Anti-FBXO43 antibody [C3], C-term used in Western Blot (WB). GTX104871

GTX104871 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX104871 diluted at 1:1000

Anti-FBXO43 antibody [C3], C-term used in Western Blot (WB). GTX104871

GTX104871 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX104871 diluted at 1:1000

Anti-FBXO43 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104871

GTX104871 IHC-P Image

FBXO43 antibody [C3], C-term detects FBXO43 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded TOV-21G xenograft.
FBXO43 antibody [C3], C-term (GTX104871) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FBXO43 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX104871

GTX104871 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Saos-2 xenograft, using FBXO43 (GTX104871) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for FBXO43 antibody [C3], C-term (GTX104871). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FBXO43 antibody [C3], C-term (GTX104871). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169