APPLICATION
Application Note
Western blot - Use at 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Calculated MW
Product Note
Reacts with residues 103-119 (EWKVEDLSRDQRKEFPN) of the human FBXO44 protein. High cross reactivity: 94% identical to mouse and rat FBG3.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
residues 103-119 (EWKVEDLSRDQRKEFPN) of the human FBXO44 protein.
Purification
Affinity purified IgG
Conjugation
Unconjugated
RRID
AB_374161
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
F-Box Protein 44 , Fbg3 , Fbx30 , Fbx6A , Fbx44 , Fbxo6A , Fbxo44
Background
F-box proteins, a critical component of the evolutionary conserved ubiquitin-protein ligase complex SCF (Skp1/Cdc53-Cullin1/F-box), recruit substrates for ubiquitination and consequent degradation through their specific protein-protein interaction domains. Novel human F-box proteins named FBG1, FBG2, FBG3, FBG4 and FBG5 constitute a third subfamily of mammalian F-box proteins. FBG genes are expressed in a limited number of human tissues including kidney, liver, brain and muscle tissues.
Research Area
DATA IMAGES
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GTX70479 WB Image
WB analysis of recombinant human FBXO44 protein using GTX70479 FBXO44 antibody. Loading : 30 μg
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REFERENCE
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REVIEW
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