APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.3-1.5μg/ml |
| 2-3μg/ml |
|
Note :
IHC-P
Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX25309-PEP).
Predict Reactivity
Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KRGSSFLAGEHPG, from the C Terminus of the protein sequence according to NP_036296.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
F-box and WD repeat domain containing 2 , FBW2 , Fwd2 , Md6
Background
F-box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins. In fact, F-box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F-box proteins. Mammalian F-box proteins are classified into three groups based on the presence of either WD-40 repeats, leucine-rich repeats, or the presence or absence of other protein-protein interacting domains. This gene encodes the second identified member of the F-box gene family and contains multiple WD-40 repeats. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX25309 IHC-P Image
IHC-P analysis of human kidney using GTX25309 FBXW2 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3μg/ml
|
|
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GTX25309 WB Image
WB analysis of human kidney lysate using GTX25309 FBXW2 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 30μg protein in RIPA buffer
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REFERENCE
There are currently no references for FBXW2 antibody, C-term (GTX25309). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for FBXW2 antibody, C-term (GTX25309). Be the first to share your experience with this product.
