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FCER1G antibody [HL1418]

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884
Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884
Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884
Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884
Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884
Anti-FCER1G antibody [HL1418] used in Western Blot (WB). GTX636884
Anti-FCER1G antibody [HL1418] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636884
Anti-FCER1G antibody [HL1418] used in Western Blot (WB). GTX636884

Cat. No. GTX636884

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1418

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Product Note

This antibody was raised against human FCER1G Intracellular domain.

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human FCER1G. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc fragment of IgE receptor Ig , FCRG

Cellular Localization

Cell membrane

Background

The high affinity IgE receptor is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat cerebellum.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded dog cerebellum.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FCER1G antibody [HL1418] used in IHC (Paraffin sections) (IHC-P). GTX636884

GTX636884 IHC-P Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FCER1G antibody [HL1418] used in Western Blot (WB). GTX636884

GTX636884 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [HL1418] (GTX636884) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FCER1G antibody [HL1418] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636884

GTX636884 ICC/IF Image

FCER1G antibody [HL1418] detects FCER1G protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FCER1G stained by FCER1G antibody [HL1418] (GTX636884) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-FCER1G antibody [HL1418] used in Western Blot (WB). GTX636884

GTX636884 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [HL1418] (GTX636884) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

FCER1G antibody [HL1418] Cat. No. GTX636884

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-Apr-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 WT and FCER1G KO cells
Amount used : 40 μg
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 24-Apr-2024
Score :
Application Tested : IP
Sample Species : Hu
Sample : THP-1 cells
Lane Description : SM: 6% starting material
UB: 6% unbound fraction
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together, 30 μL of protein A-Sepharose beads. 0.75 mL aliquots at 0.8 mg/mL of lysate were incubated, an antibody-bead conjugate for ~1 hrs at 4ºC.

Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Package List Price ($)
$ 399
$ 169
Custom Conjugations