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FCER1G antibody [N2C3]

KO/KD Orthogonal Overexpression
Anti-FCER1G antibody [N2C3] used in Western Blot (WB). GTX108487
Anti-FCER1G antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108487
Anti-FCER1G antibody [N2C3] used in Western Blot (WB). GTX108487
Anti-FCER1G antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108487
Anti-FCER1G antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108487
Anti-FCER1G antibody [N2C3] used in Western Blot (WB). GTX108487

Cat. No. GTX108487

2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all FCER1G products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human FCER1G. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950266

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc fragment of IgE receptor Ig , FCRG

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The high affinity IgE receptor is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha, 1 beta, and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FCER1G antibody [N2C3] used in Western Blot (WB). GTX108487

GTX108487 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] (GTX108487) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-FCER1G antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108487

GTX108487 ICC/IF Image

FCER1G antibody [N2C3] detects FCER1G protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FCER1G stained by FCER1G antibody [N2C3] (GTX108487) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-FCER1G antibody [N2C3] used in Western Blot (WB). GTX108487

GTX108487 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] (GTX108487) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FCER1G antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108487

GTX108487 ICC/IF Image

FCER1G antibody [N2C3] detects FCER1G protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FCER1G stained by FCER1G antibody [N2C3] (GTX108487) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-FCER1G antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108487

GTX108487 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U873 xenograft, using FCER1G(GTX108487) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FCER1G antibody [N2C3] used in Western Blot (WB). GTX108487

GTX108487 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] (GTX108487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

FCER1G antibody [N2C3] Cat. No. GTX108487

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-FCER1G antibody [N2C3] used in Western Blot (WB). GTX108487
Date : Anonymous submitted on 24-Apr-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : THP-1 WT and FCER1G KO cells
Amount used : 40 μg
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-FCER1G antibody [N2C3] used in Immunoprecipitation (IP). GTX108487
Date : Anonymous submitted on 24-Apr-2024
Score :
Application Tested : IP
Sample Species : Hu
Sample : THP-1 cells
Primary Antibody :
Lane Description : SM: 6% starting material
UB: 6% unbound fraction
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together, 30 μL of protein A-Sepharose beads. 0.75 mL aliquots at 0.8 mg/mL of lysate were incubated, an antibody-bead conjugate for ~1 hrs at 4ºC.

Data provided by YCharOS Inc., an open science company, the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169