Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] (GTX108487) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] (GTX108487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded U873 xenograft, using FCER1G(GTX108487) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

FCER1G antibody [N2C3] detects FCER1G protein at cell membrane by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FCER1G stained by FCER1G antibody [N2C3] (GTX108487) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] (GTX108487) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

FCER1G antibody [N2C3] detects FCER1G protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FCER1G stained by FCER1G antibody [N2C3] (GTX108487) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).