GeneTex
United States (US)

FCP1 antibody [N1N2], N-term

GTX115194_40541_WB_18121410_749.jpg
GTX115194_40541_20150324_IFA_18121410_258.jpg

Cat No. GTX115194

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Zebrafish, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human FCP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CCFDN antibody, FCP1 antibody, CTDP1 antibody, transcription factor IIF-associating CTD phosphatase 1 antibody, RNA polymerase II subunit A C-terminal domain phosphatase antibody, "CTD of POLR2A, phosphatase of, subunit 1 antibody", serine phosphatase FCP1a antibody, TFIIF-associating CTD phosphatase 1 antibody, "CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) phosphatase, subunit 1 antibody"

Cellular Localization

Nucleus

Background

This gene encodes a protein which interacts with the carboxy-terminus of transcription initiation factor TFIIF, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. The protein may also represent a component of an RNA polymerase II holoenzyme complex. Alternative splicing of this gene results in two transcript variants encoding 2 different isoforms. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115194_40541_WB_18121410_749.jpg

GTX115194 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX115194 diluted at 1:1000

GTX115194_40541_20150324_IFA_18121410_258.jpg

GTX115194 ICC/IF Image

FCP1 antibody [N1N2], N-term detects FCP1 protein at nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FCP1 protein stained by FCP1 antibody [N1N2], N-term (GTX115194) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
Glycerol.pdf
Thimerosal.pdf