WB analysis of membrane enriched extracts (30 μg lysate) of U-87 MG (Lane 1), SH-SY5Y (Lane 2), NTERA-2 (Lane 3), U-2 OS (Lane 4), A549 (Lane 5), Mouse Brain (Lane 6) and PC-12 (Lane 7) using GTX25668 FE65 antibody.
Dilution : 1-2 μg/ml

WB analysis of pulldown material form mouse brain lysate using GTX25668 FE65 antibody.
Lane 1 : an immunopurified sample from a full-length Fe65 Knockout mouse
Lane 2 : an immunopurified sample from an Fe65 wild type mouse

ICC/IF analysis of SH-SY5Y cells using GTX25668 FE65 antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature