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FEN1 antibody [4E7]

Anti-FEN1 antibody [4E7] used in Western Blot (WB). GTX70185
Anti-FEN1 antibody [4E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70185

Cat No. GTX70185

Host

Mouse

Clonality

Monoclonal

Clone Name

4E7

Isotype

IgG1

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

MCF7

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Flap Structure-Specific Endonuclease 1 , Fen-1 , Mf1 , Rad2 , Fen1

Cellular Localization

Nuclear

Background

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 a protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Database

Research Area

DATA IMAGES

Anti-FEN1 antibody [4E7] used in Western Blot (WB). GTX70185

GTX70185 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody (GTX70185) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-FEN1 antibody [4E7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70185

GTX70185 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed MCF7, using FEN1 [4E7](GTX70185) antibody at 1:200 dilution.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 329