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FEN1 antibody [7H8]

Anti-FEN1 antibody [7H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66841
Anti-FEN1 antibody [7H8] used in Western Blot (WB). GTX66841

Cat. No. GTX66841

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7H8

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Hamster, Monkey
Package
100 μl ($459)
See all FEN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:1000-500
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.2% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human FEN-1 protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

flap structure-specific endonuclease 1 , FEN-1 , MF1 , RAD2

Cellular Localization

Nucleus, nucleoplasm

Background

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FEN1 antibody [7H8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66841

GTX66841 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX66841 FEN1 antibody [7H8].
Dilution : 1:400

Anti-FEN1 antibody [7H8] used in Western Blot (WB). GTX66841

GTX66841 WB Image

WB analysis of HeLa, Jurkat, 3T3, COS7, PC-12, C6, Raw264.7, Raji, and CHO-K1 cell lysates using GTX66841 FEN1 antibody [7H8].
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459