GeneTex
United States (US)

FEN1 antibody

GTX101777_40093_IP_19011506_691.jpg
GTX101777_40093_20141122_IHC_19011506_395.jpg
GTX101777_40093_WB_M_19011506_870.jpg
GTX101777_IFA_19011506_683.jpg
GTX101777_40093_WB_R_19011506_206.jpg
GTX101777_WB_19011506_948.jpg

Cat No. GTX101777

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, PLA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HepG2 , NIH-3T3 , JC , BCL-1 , Raw264.7 , PC-12

Predict Reactivity

Sheep, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Flap Structure-Specific Endonuclease 1,Fen-1,Mf1,Rad2,Fen1

Cellular Localization

Nucleus

Background

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101777_40093_IP_19011506_691.jpg

GTX101777 IP Image

FEN1 antibody immunoprecipitates FEN1 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 30 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of FEN1 protein by 4 μg FEN1 antibody (GTX101777)
10 % SDS-PAGE
The immunoprecipitated FEN1 protein was detected by FEN1 antibody (GTX101777) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX101777_40093_20141122_IHC_19011506_395.jpg

GTX101777 IHC-P Image

FEN1 antibody detects FEN1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FEN1 antibody (GTX101777) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101777_40093_WB_M_19011506_870.jpg

GTX101777 WB Image

FEN1 antibody detects FEN1 protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell lysate/extract
B. 30 µg JC whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysate/extract
D. 30 µg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
FEN1 antibody (GTX101777) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101777_IFA_19011506_683.jpg

GTX101777 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using FEN1(GTX101777) antibody at 1:200 dilution.

GTX101777_40093_WB_R_19011506_206.jpg

GTX101777 WB Image

FEN1 antibody detects FEN1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
10% SDS-PAGE
FEN1 antibody (GTX101777) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101777_WB_19011506_948.jpg

GTX101777 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX101777 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169