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FEN1 antibody

Anti-FEN1 antibody used in Immunoprecipitation (IP). GTX101777
Anti-FEN1 antibody used in Western Blot (WB). GTX101777
Anti-FEN1 antibody used in Western Blot (WB). GTX101777
Anti-FEN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101777
Anti-FEN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101777
Anti-FEN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101777
Anti-FEN1 antibody used in Western Blot (WB). GTX101777

Cat. No. GTX101777

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC, PLA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all FEN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
IHC Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , Raw264.7 , PC-12

Predict Reactivity

Sheep, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FEN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950269

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

flap structure-specific endonuclease 1 , FEN-1 , MF1 , RAD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FEN1 antibody used in Immunoprecipitation (IP). GTX101777

GTX101777 IP Image

FEN1 antibody immunoprecipitates FEN1 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 30 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of FEN1 protein by 4 μg FEN1 antibody (GTX101777)
10 % SDS-PAGE
The immunoprecipitated FEN1 protein was detected by FEN1 antibody (GTX101777) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-FEN1 antibody used in Western Blot (WB). GTX101777

GTX101777 WB Image

FEN1 antibody detects FEN1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10% SDS-PAGE
FEN1 antibody (GTX101777) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FEN1 antibody used in Western Blot (WB). GTX101777

GTX101777 WB Image

FEN1 antibody detects FEN1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
FEN1 antibody (GTX101777) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-FEN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101777

GTX101777 ICC/IF Image

FEN1 antibody detects FEN1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: FEN1 stained by FEN1 antibody (GTX101777) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-FEN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101777

GTX101777 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using FEN1(GTX101777) antibody at 1:200 dilution.

Anti-FEN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101777

GTX101777 IHC-P Image

FEN1 antibody detects FEN1 protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
FEN1 antibody (GTX101777) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FEN1 antibody used in Western Blot (WB). GTX101777

GTX101777 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with FEN1 antibody (GTX101777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Ying Ma et al. Antioxidants (Basel) 2023; 12 (2) : 276 RNA G-Quadruplex within the 5'-UTR of FEN1 Regulates mRNA Stability under Oxidative Stress
Application : WB
Reactivity : Human
Zeng X et al. FASEB J 2019; : fj201900273R FEN1 mediates miR-200a methylation and promotes breast cancer cell growth via MET and EGFR signaling.
Application : WB, IHC
Reactivity : Human
Szczesny B et al. Sci Rep 2016; 6 : 36125 Inhibition of hydrogen sulfide biosynthesis sensitizes lung adenocarcinoma to chemotherapeutic drugs by inhibiting mitochondrial DNA repair and suppressing cellular bioenergetics.
Application : PLA
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169