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FEN1 antibody

Anti-FEN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32600
Anti-FEN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32600
Anti-FEN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32600
Anti-FEN1 antibody used in Western Blot (WB). GTX32600
Anti-FEN1 antibody used in Immunoprecipitation (IP). GTX32600
Anti-FEN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32600

Cat No. GTX32600

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:10-1:100
IHC-P 1:50-1:200
IP 1:50-1:200
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN1 (NP_004102.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

flap structure-specific endonuclease 1 , FEN-1 , MF1 , RAD2

Cellular Localization

nucleoplasm

Background

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FEN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32600

GTX32600 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32600 FEN1 antibody.

Anti-FEN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32600

GTX32600 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX32600 FEN1 antibody.
Dilution : 1:100

Anti-FEN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32600

GTX32600 ICC/IF Image

ICC/IF analysis of A549 cells using GTX32600 FEN1 antibody.

Anti-FEN1 antibody used in Western Blot (WB). GTX32600

GTX32600 WB Image

WB analysis of various sample lysates using GTX32600 FEN1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-FEN1 antibody used in Immunoprecipitation (IP). GTX32600

GTX32600 IP Image

IP analysis of HeLa cells using GTX32600 FEN1 antibody.
IP reaction : 200μg extracts / 1μg antibody antibody
Dilution : 1:1000

Anti-FEN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX32600

GTX32600 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX32600 FEN1 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329