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FEN1 antibody

Anti-FEN1 antibody used in Western Blot (WB). GTX70184

Cat. No. GTX70184

Host

 Mouse

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all FEN1 products

APPLICATION

Application Note

For WB: Use at an assay dependent dilution. Predicted molecular weight: 43 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

43 kDa. ( Note )

Positive Control

MCF7

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human FEN-1 protein.

Purification

Unpurified
Antiserum

Conjugation

Unconjugated

RRID

AB_370521

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

flap structure-specific endonuclease 1 , FEN-1 , MF1 , RAD2

Cellular Localization

nucleoplasm

Background

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FEN1 antibody used in Western Blot (WB). GTX70184

GTX70184 WB Image

Detecion of human FEN1 protein in HeLa whole cell lysate using GeneTex polyclonal antiserum GTX70184

REFERENCE

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REVIEW

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Package List Price ($)
$ 399