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FEN1 antibody

Anti-FEN1 antibody used in Western Blot (WB). GTX70257

Cat No. GTX70257

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

For WB: Use at a dilution of 1:2,500. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLa, Raji, 3T3

PROPERTIES

Form

Liquid

Buffer

Antiserum diluted in PBS pH 7.4, containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human FEN-1 protein

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Flap Structure-Specific Endonuclease 1 , Fen-1 , Mf1 , Rad2 , Fen1

Cellular Localization

Nuclear

Background

FEN-1 (Flap EndoNuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease when it cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair, and is required in cell-free DNA replication systems.

Database

Research Area

DATA IMAGES

Anti-FEN1 antibody used in Western Blot (WB). GTX70257

GTX70257 WB Image

Western blot analysis of FEN1 expression in Raji (A) and NIH-3T3 (B) whole cell lysates.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329