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FGD4 antibody [N1N3]

Anti-FGD4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX109859
Anti-FGD4 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109859
Anti-FGD4 antibody [N1N3] used in Western Blot (WB). GTX109859
Anti-FGD4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX109859

Cat. No. GTX109859

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all FGD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

Positive Control

Molt-4

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FGD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950270

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FYVE, RhoGEF and PH domain containing 4 , CMT4H , FRABP , ZFYVE6

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection , filopodium

Background

This gene encodes a protein that is involved in the regulation of the actin cytoskeleton and cell shape. This protein contains an actin filament-binding domain, which together with its Dbl homology domain and one of its pleckstrin homology domains, can form microspikes. This protein can activate MAPK8 independently of the actin filament-binding domain, and it is also involved in the activation of CDC42 via the exchange of bound GDP for free GTP. The activation of CDC42 also enables this protein to play a role in mediating the cellular invasion of Cryptosporidium parvum, an intracellular parasite that infects the gastrointestinal tract. Mutations in this gene can cause Charcot-Marie-Tooth disease type 4H (CMT4H), a disorder of the peripheral nervous system. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FGD4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX109859

GTX109859 IHC-P Image

FGD4 antibody [N1N3] detects FGD4 protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
FGD4 antibody [N1N3] (GTX109859) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-FGD4 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109859

GTX109859 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FGD4(GTX109859) antibody at 1:200 dilution.

Anti-FGD4 antibody [N1N3] used in Western Blot (WB). GTX109859

GTX109859 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX109859 diluted at 1:1000

Anti-FGD4 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX109859

GTX109859 IHC-P Image

FGD4 antibody [N1N3] detects FGD4 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
FGD4 antibody [N1N3] (GTX109859) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169