GeneTex
United States (US)

FGF13 antibody

GTX101008_40723_IHC_19011506_914.jpg
GTX101008_40723_20150821_IHC-P_M_19011506_646.jpg
GTX101008_40723_IHC_M_19011506_942.jpg
GTX101008_40723_WB_19011506_547.jpg
GTX101008_40723_IHC_R_19011506_481.jpg

Cat No. GTX101008

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human FGF13. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Fibroblast Growth Factor 13,Fgf-13,Fgf2,Fhf-2,Fhf2,Fgf13

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This gene is located in a region on chromosome X, which is associated with Borjeson-Forssman-Lehmann syndrome (BFLS), making it a possible candidate gene for familial cases of the BFLS, and for other syndromal and nonspecific forms of X-linked mental retardation mapping to this region. Alternative splicing of this gene at the 5' end results in several transcript variants encoding different isoforms with different N-termini. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101008_40723_IHC_19011506_914.jpg

GTX101008 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using FGF13(GTX101008) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101008_40723_20150821_IHC-P_M_19011506_646.jpg

GTX101008 IHC-P Image

FGF13 antibody detects FGF13 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
FGF13 antibody (GTX101008) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101008_40723_IHC_M_19011506_942.jpg

GTX101008 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using FGF13(GTX101008) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101008_40723_WB_19011506_547.jpg

GTX101008 WB Image

Sample (30 µg of whole cell lysate)
A: MCF-7
12% SDS PAGE
GTX101008 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101008_40723_IHC_R_19011506_481.jpg

GTX101008 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using FGF13(GTX101008) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169