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FGF2 antibody [3D9]

Overexpression
Anti-FGF2 antibody [3D9] used in IHC (Paraffin sections) (IHC-P). GTX84502
Anti-FGF2 antibody [3D9] used in Western Blot (WB). GTX84502
Anti-FGF2 antibody [3D9] used in Western Blot (WB). GTX84502
Anti-FGF2 antibody [3D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84502

Cat. No. GTX84502

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D9

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Monkey
Package
100 μl ($539)
See all FGF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10727913

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibroblast growth factor 2 , BFGF , FGF-2 , FGFB , HBGF-2

Cellular Localization

Secreted,Nucleus

Background

The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Database

Research Area

DATA IMAGES

Anti-FGF2 antibody [3D9] used in IHC (Paraffin sections) (IHC-P). GTX84502

GTX84502 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84502 FGF2 antibody [3D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FGF2 antibody [3D9] used in Western Blot (WB). GTX84502

GTX84502 WB Image

Overexpression

WB analysis of HEK293T cells transfected with FGF2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84502 FGF2 antibody [3D9].
Loading : 5 ug per lane

Anti-FGF2 antibody [3D9] used in Western Blot (WB). GTX84502

GTX84502 WB Image

WB analysis of various cell lines using GTX84502 FGF2 antibody [3D9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-FGF2 antibody [3D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84502

GTX84502 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with FGF2 plasmid using GTX84502 FGF2 antibody [3D9].

REFERENCE

Application Reference

Huang H et al. Diabetes Metab Syndr Obes 2020; 13 : 2279-2288 Role of TNF-α and FGF-2 in the Fracture Healing Disorder of Type 2 Diabetes Model Induced by High Fat Diet Followed by Streptozotocin.
Application : IHC-P
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539