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FGF2 antibody [3D9]

Anti-FGF2 antibody [3D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84502
Anti-FGF2 antibody [3D9] used in IHC (Paraffin sections) (IHC-P). GTX84502
Anti-FGF2 antibody [3D9] used in Western Blot (WB). GTX84502
Anti-FGF2 antibody [3D9] used in Western Blot (WB). GTX84502

Cat. No. GTX84502

Host

Mouse

Clonality

Monoclonal

Clone Name

3D9

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Monkey
Package
100 μl ($539)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 10-155 of human bFGF (NP_001997) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_10727913

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibroblast growth factor 2 , BFGF , FGF-2 , FGFB , HBGF-2

Cellular Localization

Secreted,Nucleus

Background

The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.

Database

Research Area

DATA IMAGES

Anti-FGF2 antibody [3D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84502

GTX84502 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with FGF2 plasmid using GTX84502 FGF2 antibody [3D9].

Anti-FGF2 antibody [3D9] used in IHC (Paraffin sections) (IHC-P). GTX84502

GTX84502 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84502 FGF2 antibody [3D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-FGF2 antibody [3D9] used in Western Blot (WB). GTX84502

GTX84502 WB Image

WB analysis of various cell lines using GTX84502 FGF2 antibody [3D9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-FGF2 antibody [3D9] used in Western Blot (WB). GTX84502

GTX84502 WB Image

WB analysis of HEK293T cells transfected with FGF2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84502 FGF2 antibody [3D9].
Loading : 5 ug per lane

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539