ELISA: This antibody can be used as a coating antibody at 10 μg/ml in a two site ELISA with biotin conjugated bFGF antibody. Typically the ELISA has a working range of 0-2000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 μl. Recovery of human basic FGF in human serum is typically > 85% in the ELISA. IHC: This antibody can be used at 1-10 ug/ml to stain human basic FGF expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10minutes at 48C, plus 0.1% saponin for 20minutes at room temperature. Optimal dilutions/concentrations should be determined by the end user.
Recognizes human basic FGF. It does not recognize human acidic FGF or a panel of other human cytokines.
0.08% Sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Protein G purified
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
fibroblast growth factor 2 , BFGF , FGF-2 , FGFB , HBGF-2
Secreted , Nucleus
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]