ELISA: This antibody can be used as a solution phase antibody at 2 μg/ml in a two site ELISA with unconjugated FGF basic antibody. Typically the ELISA has a working range of 0-2000 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recovery of human basic FBF is human serum is typically >85% in the ELISA. Optimal dilutions/concentrations should be determined by the end user.
Does not recognise human acidic FGF or a panel of other human cytokines
Preservative: 0.08% Sodium Azide; Constituents: PBS
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Unfortunately, this information is considered to be commercially sensitive
Protein G purified
Antibody Purity > 95 % by SDS PAGE
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Fibroblast Growth Factor 2,Bfgf,Fgf-2,Fgfb,Hbgf-2,Fgf2
FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.