APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:200 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 143-288 of human FGF2 (NP_001997.5).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
fibroblast growth factor 2 , BFGF , FGF-2 , FGFB , HBGF-2
Cellular Localization
Secreted,Nucleus
Background
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX64354 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX64354 FGF2 antibody. Dilution : 1:100
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GTX64354 IHC-P Image
IHC-P analysis of human colon tissue using GTX64354 FGF2 antibody. Dilution : 1:100
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GTX64354 ICC/IF Image
ICC/IF analysis of C6 cells using GTX64354 FGF2 antibody. Blue : DAPI Dilution : 1:100
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GTX64354 ICC/IF Image
ICC/IF analysis of L929 cells using GTX64354 FGF2 antibody. Blue : DAPI Dilution : 1:100
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GTX64354 IHC-P Image
IHC-P analysis of rat ovary tissue using GTX64354 FGF2 antibody. Dilution : 1:100
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GTX64354 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX64354 FGF2 antibody. Blue : DAPI Dilution : 1:100
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GTX64354 WB Image
WB analysis of 293T cell lysates using GTX64354 FGF2 antibody. Dilution : 1:1000 Loading : 25 μg per lane
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REFERENCE
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REVIEW
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