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FGF2 antibody

Anti-FGF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64354
Anti-FGF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64354
Anti-FGF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64354
Anti-FGF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64354
Anti-FGF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64354
Anti-FGF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64354
Anti-FGF2 antibody used in Western Blot (WB). GTX64354

Cat. No. GTX64354

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all FGF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 143-288 of human FGF2 (NP_001997.5).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibroblast growth factor 2 , BFGF , FGF-2 , FGFB , HBGF-2

Cellular Localization

Secreted,Nucleus

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-FGF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64354

GTX64354 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64354 FGF2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-FGF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64354

GTX64354 IHC-P Image

IHC-P analysis of human colon tissue using GTX64354 FGF2 antibody.
Dilution : 1:100

Anti-FGF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64354

GTX64354 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64354 FGF2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-FGF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64354

GTX64354 IHC-P Image

IHC-P analysis of rat ovary tissue using GTX64354 FGF2 antibody.
Dilution : 1:100

Anti-FGF2 antibody used in IHC (Paraffin sections) (IHC-P). GTX64354

GTX64354 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX64354 FGF2 antibody.
Dilution : 1:100

Anti-FGF2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64354

GTX64354 ICC/IF Image

ICC/IF analysis of C6 cells using GTX64354 FGF2 antibody.
Blue : DAPI
Dilution : 1:100

Anti-FGF2 antibody used in Western Blot (WB). GTX64354

GTX64354 WB Image

WB analysis of 293T cell lysates using GTX64354 FGF2 antibody.
Dilution : 1:1000
Loading : 25 μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399