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FGF21 antibody [N3C3]

Anti-FGF21 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111877
Anti-FGF21 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111877
Anti-FGF21 antibody [N3C3] used in Western Blot (WB). GTX111877

Cat No. GTX111877

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

DDDDK-tagged FGF21-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FGF21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950272

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

fibroblast growth factor 21

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-FGF21 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111877

GTX111877 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using FGF21(GTX111877) antibody at 1:200 dilution.

Anti-FGF21 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111877

GTX111877 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HS578T xenograft, using FGF21(GTX111877) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-FGF21 antibody [N3C3] used in Western Blot (WB). GTX111877

GTX111877 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with FGF21 antibody [N3C3] (GTX111877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for FGF21 antibody [N3C3] (GTX111877). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for FGF21 antibody [N3C3] (GTX111877). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf